Definition of Combinatorial Testing
In this scheme, three or more reagents react such that each reagent is incorporated into the final product in a single step, eliminating the need for a multi-step synthesis that involves many purification steps. In MCCs, there is no deconvolution required to determine which compounds are biologically-active because each synthesis in an array has only a single product, thus the identity of the compound should be unequivocally known. In computer science, all-pairs testing or pairwise testing is a combinatorial method of software testing that, for each pair of input parameters to a system , tests all possible discrete combinations of those parameters. Using carefully chosen test vectors, this can be done much faster than an exhaustive search of all combinations of all parameters, by « parallelizing » the tests of parameter pairs.
They used infrared thermography to identify catalysts in non-peptide tethered libraries. The method is based on the heat that is evolved in the beads that contain a catalyst when the tethered library immersed into a solution of a substrate. When the beads are examined through an infrared microscope the catalyst containing beads appear as bright spots and can be picked out.
In its modern form, combinatorial chemistry has probably had its biggest impact in the pharmaceutical industry. Researchers attempting to optimize the activity profile of a compound create a ‘library’ of many different but related compounds. Advances in robotics have led to an industrial approach to combinatorial synthesis, enabling companies to routinely produce over 100,000 new and unique compounds per year. The most common bugs in a program are generally triggered by either a single input parameter or an interaction between pairs of parameters.
Combinatorial testing is a method that can reduce cost [...]